Antimicrobial and Cytotoxic Properties of Extracts from Over-seasoned Worm-casts of the Earthworm Hyperiodrilus Africanus Beddard, 1891

⦁ Background: Despite the continuous interest in search for therapeutic agents, little attention has been given to medicinal relevance of earthworm casts, with even less over-seasoned worm-casts. Therefore, this study determined phytochemical, antimicrobial, and cytotoxic properties worm-casts Hyperiodrilus africanus (Eudrilidae). Methods: The casts were extracted n-hexane, ethanol, water crude extracts evaluated presence chemical constituents antimicrobial properties. Cytotoxicity was inferred from antimitotic effects on radicles germinating seeds Sorghum bicolor. Results: constituent determinations revealed alkaloids, anthraquinones, coumarins, steroids, terpenoids, tannins, cardiac glycosides, phenols. Screening that ethanolic aqueous had more than n-hexane extracts. extract showed antibacterial activity against Streptococcus sp. Staphylococcus aureus; antifungal Aspergillus flavus niger. Furthermore, both healthy cells S. bicolor a manner similar reference drug (cyclophosphamide). Conclusion: provides evidence, lending credence potential H. africanus.